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Injection of DNA nucleosides resulting from enzymatic hydrolysis of AFB1-DNA (11/05/1975)
Purpose/Intro: To test enzymatic hydrolysis as an alternative to acidic hydrolysis for liberating the adduct from DNA.
Results/Conclusion: Not a very clean chromatogram.
Notes: Enzymatic DNA hydrolysis was an alternative to acidic hydrolysis and it was commonly used at the time. It was carried out in basic (pH ~ 8) conditions. Since aflatoxin is sensitive to base, John concentrated his efforts on acid hydrolysis—but tried enzymatic just in case.
Links:
PDF: See this in the notebook!
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