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Interlaced Optical Force-Fluorescence Measurements for Single Molecule Biophysics
R R Brau
P B Tarsa
M J Lang
J M Ferrer
Research question: How does one combine optical tweezer force spectroscopy and single molecule flourescence spectroscopy to study a biological molecule's force exertion and configuration changes?
An optical trap.
Optical trapping systems are used to study the forces involved in the functioning of
various biological molecules, such as DNA, enzymes, and viruses. Such molecules can also be
studied with flourescence microscopy, which serves to elucidate their configurational changes.
Professor Matthew Lang and his team have devised a method that combines both techniques so
that one can study a molecule's structure and forces simultaneously. The group's work is
described in their paper, Interlaced Optical Force-Fluorescence Measurements for Single
Molecule Biophysics. Their paper and their technique will be discussed in detail in this
This module contains:
- An overview that discusses the research and provides some background on the technology and equipment involved.
- A detailed experiment timeline. Selected experiments are linked
to audiovisual supplementary
- Video interviews with Matthew Lang, Ricardo Brau, Jorge Ferrer, and Peter Tarsa. The authors explain
experience and their future plans.
- Author profiles for Matthew Lang, Ricardo Brau, Jorger Ferrer, and Peter Tarsa (click on the links at the top of this page).
- The full manuscript (HTML and PDF).
- A glossary of some obscure terms. The glossary is also accessible from the Read the Paper page. Move the mouse over any hyperlinked word and its definition will appear
in a tooltip.