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HPLC analysis of butanol extract (03/01/1976)
Purpose/Intro: "The previous pages describe an experiment in which it was demonstrated that radioactivity covalently bound to a base was extracted with butanol. The sample (11-3-1) still has ~200 µL of BuOH in the lower phase. This will be concentrated and analyzed by HPLC."
Results/Conclusion: "Nice chromatogram with about 3-4 large peaks of 364 nm-absorbing material. The radioactivity was primarily in 2 of the peaks. Two large 254-absorbing peaks were observed. Conclusions: Generally, the results are somewhat encouraging. Base(?)-AFB1 adducts were separated well from 254-nm absorbing material. The reason that 2 major adducts were seen could be that there was chemical degradation or that there are actually 2 present—analysis of nucleoside adducts under these conditions shows only a single peak with radioactivity. The rest of the 3H primarily elutes early in the chromatogram and may be unhydrolyzed adducts which are qualitatively different from the late-eluting compound."
Links:
PDF: See this in the notebook!
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