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Perchloric acid hydrolysis of Peak 1 (04/20/1976)

Purpose/Intro: To determine what base(s) (adenine, guanine, cytosine, thymine) are present in the putative adduct, Peak 1.
Results/Conclusion: "A small peak was seen in the hydrolysate at the retention time of guanine. These results were reproducible and peaks were not seen at the tRs of cytosine and adenine. Conclusions: 1. There is a peak at the tR of guanine after HClO4 hydrolysis of the presumptive adduct in Peak 1; 2. Can't make any generalizations, but there was a large peak early in chromatogram—this usually occurs, however, and no thymine adduct has ever been identified. Cytosine and adenine were not observed."

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