You are here: MIT OpenLabWare » Aflatoxin » Read the Notebook
Read the Notebook


Page 55 of 204
Retrieve full-size image (3392x4400, 488KB) | Next Page >>


Hydrolysis of in vivo adducted DNA (06/09/1976)

Purpose/Intro: "RC and WFB injected 1 mg/kg AFB1-3H (50 (?)) into a rat and waited 2 hours before killing. Liver and kidney DNA are being prepared by the Kirby procedure. Since the HCOOH technique has been so successfully applied to efficient and facile adduct liberation, it will be used to see if in vivo adduct can be observed bound to DNA."
Results/Conclusion: "Three peaks of A365 were observed in the adduct region. Fractions were collected. This may be very promising. This DNA wasn't pure, of course—it may have been contaminated with RNA, protein and AFB1. The aflatoxin possibly was degraded to B which may represent the peaks we observe."

Links: 
PDF: See this in the notebook!
 
MIT Massachusetts Institute of Technology © 2007 MIT | Legal Notices | Contact Us Creative Commons License