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Hydrolysis of in vivo adducted DNA (06/09/1976)
Purpose/Intro: "RC and WFB injected 1 mg/kg AFB1-3H (50 (?)) into a rat and waited 2 hours before killing. Liver and kidney DNA are being prepared by the Kirby procedure. Since the HCOOH technique has been so successfully applied to efficient and facile adduct liberation, it will be used to see if in vivo adduct can be observed bound to DNA."
Results/Conclusion: "Three peaks of A365 were observed in the adduct region. Fractions were collected. This may be very promising. This DNA wasn't pure, of course—it may have been contaminated with RNA, protein and AFB1. The aflatoxin possibly was degraded to B2α which may represent the peaks we observe."
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PDF: See this in the notebook!
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