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JE-4 DNA: 10-9-1 processing (10/11/1976)
Purpose/Intro: To isolate more of the adduct to determine its structure
Results/Conclusion: "Publishable chromatogram."
Notes: This is when John forgot to set the gradient before going to lunch. As a result, the system kept running at 10% methanol for several hours. Despite running for so long, the adduct remained on the column—after the 254 nm-absorbing material had been washed off. When John ran the gradient, the adduct eluted at the same retention time, indicating that it had stayed at the start of the column for all that time.
Links:
PDF: See this in the notebook!
Video: The Two Liter Problem Part I
Video: The Two Liter Problem Part II
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