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Preparative column for adduct purification (10/18/1976 - 10/19/1976)
Purpose/Intro: "A preparative reversed phase column was made to purify the adduct from other DNA components."
Results/Conclusion: "The adduct had a tR of 32 min and gave a symmetrical peak. These should be good conditions to try for its isolation."
Notes: Two ideas resulted from the previous experiment. The first is that multiple injections could be done before running the gradient, thus accumulating material on the column. The second is that the column could be hooked up directly to a pump, thus getting around the 1 mL injection limit of the HPLC system. Both of these solutions allow a lot of adduct to build up at the start of the column, thus achieving a high concentration in the collected peak and getting around the problem of evaporating 2 L of water.
Links:
PDF: See this in the notebook!
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