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Preparative run on 25 mL of 10-21-1 (JE-4 DNA) (10/21/1976)
Purpose/Intro: "The prep run on the adduct done earlier in the day worked well, so 25 mL of the new sample will be processed now (this is < 25% of entire 10-21-1 sample). In terms of the amount of DNA represented, this is probably in excess of 20 mg."
Results/Conclusion: "1. 254 was off-scale much of the time; 2. the middle peak is building up on the prep column."
Notes: "Oligon pk" means "oligonucleotide peak" and is the large peak immediately before the 32 min adduct peak. "254" refers to "254 nm-absorbing material," in other words, the four DNA bases.
Links:
PDF: See this in the notebook!
Video: The "Go for it all" Story
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