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- QuickTime: A Guided Tour of Optical Trapping
Matt gives a guided tour of an optical trap in his lab.
- QuickTime: Advice to High School Students
Matt Lang gives high school students advice on pursuing science as a career.
- QuickTime: Applications of Optical Traps
Matt talks about some of the applications of optical traps.
- QuickTime: As a postdoc, how do you know when you're done?
Peter explains how a postdoc term
ends.
- QuickTime: Can you tell us the goal of this portion of the paper?
Peter explains the goal of this
section of the project.
- QuickTime: Do you work alone in the lab?
Peter explains the work
environment in Matt Lang's lab.
- QuickTime: Do you work in a chemistry, biology, or physics lab?
Ricardo answers the unanswerable.
- QuickTime: Do you work in a chemistry, biology, or physics lab?
Peter answers the unanswerable.
- QuickTime: Future Plans
Ricardo talks about his future plans.
- QuickTime: Future Plans for the Project
Ricardo talks about where this project is going, post-publication.
- QuickTime: How did you become interested in science?
Peter discusses his first steps
in a scientific career path.
- QuickTime: How did you get into science?
Matt Lang describes his background, how he got interested in science, and how he came to MIT.
- QuickTime: How do you choose the right journal for publication?
Jorge explains how one selects
a journal that is fitting to
one's research.
- QuickTime: How do you like it at MIT?
Jorge talks about his MIT
experience.
- QuickTime: How often do experiments actually work?
Peter describes how frequently
his experiments are successful
and how he proceeds after gathering
positive data.
- QuickTime: How reproduceable are your experiments?
Jorge discusses how other
scientists may repeat their
research.
- QuickTime: How was trap stiffness determined?
Ricardo explains how the
Equipartition Theorem can be
used to determine the strength
of an optical trap.
- QuickTime: Is your lab a Biology, Chemistry, or Physics Lab?
Matt Lang answers the unanswerable.
- QuickTime: Is your work boring?
Peter shoots down the question.
- QuickTime: Jorge's background
Jorge talks about his previous
experiences.
- QuickTime: Mentors
Matt Lang talks about his mentors.
- QuickTime: Nail Polish
Matt explains how (and why) his lab uses nail polish.
- QuickTime: Optical Modulation frequency
Ricardo discusses how they
determined how often the optical
trap laser needs to be turned on
and off.
- QuickTime: Optical Trap Demo -- MIT spelled using E. Coli
Seventeen time-shared optical traps hold seventeen E. Coli cells in a familiar shape...
David Appleyard, graduate student in the Lang Group, explains what "time-sharing" means in the world of optical traps: "My favorite way to
describe it is to use the analogy of someone juggling using those plates
which you balance on the end of a stick by spinning them real fast. If a
juggler wants to keep 3 plates balanced, he spins each one individually, and
then continuously returns to each plate individually to give it a little
extra push to keep its' speed and balance. Same idea with the trap. We
have one laser beam which is alternated between all of the objects that we
want to keep in position. As long as the laser beam returns to each
object before the object has had a moment to diffuse away, the object will
stay in position. We just cycle the laser between all the positions as
fast as possible (with our instrument, this is about 1000 Hz - and for 5 or
6 objects, that gives about 200 times per second)."
- QuickTime: Optical Trap Demo -- Six two-micron particles
This trap is unusual because the manipulation is being done through silicon rather than a glass slide.
- QuickTime: Optical Trap Demo -- Spinning E. Coli
E. Coli bacteria are stuck to a slide, spinning around. An optical trap is turned on and is used to halt the rotation of a single E. Coli cell.
- QuickTime: Optical Trap Demo -- Twenty-micron square polymer
A twenty-micron square particle is rotated with four optical traps.
- QuickTime: Peter's background
Peter discusses his educational
and work experience.
- QuickTime: Pros and Cons
Matt talks about the pros and cons of working a field like Biological Engineering?
- QuickTime: Ricardo's Background
Ricardo talks about his background, how he got to MIT, and whether he likes it here.
- QuickTime: The DNA results
Jorge discusses the combined
measurement results.
- QuickTime: The Impact of the Key Innovation
Matt Lang describes why the key innovation is important.
- QuickTime: The Key Innovation
Here, Matt Lang explains the key innovation that allowed his group to combine fluorescence and force measurements.
- QuickTime: The major stumbling block for combining force and fluorescence
Ricardo discusses the major stumbling block when it came to combining the optical trapping and fluorescence measurement techniques.
- QuickTime: The peer review process
Jorge talks about the critiques
and reviews of research papers.
- QuickTime: The writing process
Jorge discusses how they
organized their data and
presented it in the paper.
- QuickTime: Trap stiffness effect by modulation frequency?
Ricardo explains that trap
stiffness increases with both
laser power and modulation
frequency.
- QuickTime: Were you confident that the paper would be published?
Jorge discusses the academic
community's interest in their
research.
- QuickTime: What advice do you have for high school students?
Jorge gives some wisdom to
students interested in science
and engineering.
- QuickTime: What are your future plans?
Jorge talks about life after MIT.
- QuickTime: What are your future plans?
Peter discusses his future career
goals.
- QuickTime: What do you do when an experiment doesn't work?
Peter explains how he deals with
failure in the laboratory.
- QuickTime: What is a typical day for you in the lab?
Peter discusses his routine.
- QuickTime: What is Photobleaching?
Ricardo explains photobleaching.
- QuickTime: What is the difference between a PhD candidate and a postdoc?
Peter explains the different
roles of a PhD candidate and a
postdoc.
- QuickTime: What is the methodology behind the DNA experiment?
Jorge explains how the DNA tether
experiment was performed.
- QuickTime: What is the task division?
Ricardo explains each person's
role in the project.
- QuickTime: What is your general approach to research?
Jorge discusses his methods for
taking on new research problems.
- QuickTime: What skills does an engineer bring to a bio lab?
Jorge explains how engineering
can be applied to biological
sciences.
- QuickTime: Why optical tweezers?
Matt explains why he uses an optical trap (as opposed to just building really small metal tweezers).
- QuickTime: Why Cy3?
Peter explains why they decided
to test Cy3.
- QuickTime: Why did you come back to MIT?
Peter explains why he decided to
return to MIT and the Boston
area.
- QuickTime: Why is it important to measure force and fluorescence at the same time?
Peter explains the significance
of their research project.
- QuickTime: Why were experiments with other dyes performed but not presented?
Peter explains the reasoning
behind presenting only the Cy3
data.