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Use of preparative column to isolate adduct (10/19/1976)
Purpose/Intro: "To evaluate the potential of the preparative column for making large quantities of adduct."
Results/Conclusion: The adduct elutes as a separable peak.
Notes: Sample 10-9-1 used. Samples 10-19-9 and 10-19-10 are the fractions collected from the adduct peak (hypothetically pure adduct). The procedure here was to load the 7 mL sample onto the preparative column using a pump, then disconnect the pump and connect the column to the Micromeritics pump (which could pump gradients), wash the column with 10% methanol until 254 nm-absorbing material is washed off, then finally elute the adduct using a gradient to 80% methanol. This differs from the precolumn method in two ways: in the earlier experiment, no Micromeritics system is used, and in the earlier experiment, no methanol wash is used.
Links:
PDF: See this in the notebook!
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